The DiI perchlorate family represents a class of highly stable lipophilic carbocyanine fluorescent dyes widely used in cell biology, neuroscience, and membrane research. These dyes, including DiIC18(3) perchlorate (CAS: 41085-99-8), DiIC12(3) perchlorate (CAS: 75664-01-6), and DiIC16(3) perchlorate (CAS: 78566-75-3 / 84109-11-5), are specifically designed for labeling cell membranes and neuronal structures due to their strong lipid affinity and photostability.
Structurally, DiI dyes consist of long alkyl chains that allow them to integrate firmly into the lipid bilayer, resulting in durable and uniform membrane labeling. Once incorporated, they exhibit intense red-orange fluorescence (excitation ~549 nm, emission ~565 nm), making them ideal for confocal microscopy, flow cytometry, and live-cell imaging.
Each variant in the DiI series offers distinct properties to suit different experimental needs:
DiIC12(3) perchlorate (CAS: 75664-01-6) - Shorter alkyl chains for faster membrane diffusion and efficient labeling in dynamic cellular systems.
DiIC16(3) perchlorate (CAS: 78566-75-3 / 84109-11-5) - Balanced hydrophobicity providing excellent photostability and retention for long-term imaging.
DiIC18(3) perchlorate (CAS: 41085-99-8) - The most commonly used variant, ensuring high signal intensity and minimal background fluorescence for precise membrane visualization.
The DiI series is particularly valuable in neuronal tracing, cell migration studies, and membrane trafficking research, where selective, non-toxic, and long-lasting staining is essential. Its compatibility with both live and fixed cells enables flexible experimental design across multiple imaging platforms.
UCHEM offers high-purity DiI perchlorate dyes with verified quality and reliable performance. All variants are available in stock and supported with technical data for research reproducibility. Whether applied in cell labeling, neuroanatomical mapping, or biomembrane analysis, UCHEM’s DiI dye series provides scientists with stable, bright, and consistent fluorescence solutions for cutting-edge imaging applications.
