HEPES CAS 7365-45-9: A Reliable Biological Buffer for Cell Culture and Biochemical Research

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Author : Nina He
Update time : 2026-07-07 15:32:57

HEPES (CAS: 7365-45-9) is one of the most widely used Good's buffers for biological and biochemical applications. It is recognized for its excellent buffering capacity near physiological pH, low metal ion binding, and minimal interference with biochemical reactions, making it an essential reagent in life science research.

 

HEPES is commonly used in cell culture media, molecular biology, protein purification, enzyme assays, immunological studies, and diagnostic reagent development. Unlike bicarbonate buffer systems, HEPES maintains stable pH even when samples are handled outside a CO₂ incubator, providing greater flexibility during laboratory procedures.

 

Thanks to its high water solubility, chemical stability, and low biological toxicity, HEPES is frequently selected for applications requiring precise pH control under physiological conditions. It is also compatible with a wide range of biological systems, making it a standard buffering reagent in research laboratories, biotechnology, and pharmaceutical development.

 

To support a broad range of laboratory and industrial applications, UCHEM also supplies a comprehensive portfolio of biological buffers, including:

MOPS CAS: 1132-61-2

PIPES CAS: 5625-37-6

BES CAS: 10191-18-1

Bicine CAS: 150-25-4

ACES CAS: 7365-82-4

CAPS CAS: 1135-40-6

CHES CAS: 103-47-9

TAPS CAS: 29915-38-6

TAPSO CAS: 68399-81-5

HEPPS CAS: 16052-06-5

Tris CAS: 77-86-1

Tricine CAS: 5704-04-1

 

Whether for routine laboratory research or commercial manufacturing, UCHEM provides consistent quality, reliable supply, and professional support for a wide range of biological buffer products.

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