5'-O-DMT-N4-Ac-dC CAS:100898-63-3 is a modified deoxynucleoside derivative widely used as a phosphoramidite monomer in DNA synthesis and molecular biology research. Its structure integrates three essential features:
N4-Acetyl protection: Prevents unwanted side reactions during solid-phase oligonucleotide synthesis, ensuring high coupling efficiency and selectivity.
5'-O-Dimethoxytrityl (DMTr) group: An acid-labile protecting group that allows efficient deprotection and purification in automated DNA synthesis.
Deoxycytidine core: Serves as a fundamental DNA building block and precursor for generating various functionalized nucleosides.
Key Applications
Oligonucleotide Synthesis: Essential for preparing research-grade and therapeutic oligonucleotides, supporting studies in gene regulation and nucleic acid drug development.
Antiviral and Anticancer Research: Used as a precursor for synthesizing modified nucleosides with potential antiviral and anticancer properties.
dod-DNA Production: Plays a crucial role in the synthesis of dodecyl phosphoramidite, a key precursor for dod-DNA.
Biochemical Studies: Applied in investigations of DNA–protein interactions and in the development of DNA-based nanomaterials.
In summary, 5'-O-DMT-N4-Ac-dC (CAS: 100898-63-3) is not only a critical building block in DNA synthesis but also an important compound for advancing therapeutic research and nanobiotechnology.
