5'-O-DMT-N4-Bz-5-Me-dC CAS:104579-03-5 is a specially modified nucleoside derivative with significant value in nucleic acid chemistry and biological research. Its structure includes three essential modifications:
5-Methylcytosine: Enhances epigenetic stability and improves resistance to enzymatic degradation, making it fundamental for studying DNA methylation and gene regulation.
N4-Benzoyl protection: Prevents unwanted side reactions during solid-phase phosphoramidite synthesis, ensuring high selectivity and purity in nucleotide coupling.
5'-O-Dimethoxytrityl (DMTr) group: An acid-labile protecting group that facilitates efficient deprotection and purification during automated DNA synthesis.
Applications
Oligonucleotide synthesis: Serves as a key intermediate for preparing oligonucleotides containing methylcytosine, widely used in gene regulation, epigenetics, and nucleic acid drug development.
Nucleic acid research tools: Improves stability and binding specificity of DNA fragments, making it valuable for probe design and molecular biology studies.
Biopharmaceutical research: As a modified monomer, it supports the preparation of DNA molecules with enhanced properties, laying the foundation for antisense oligonucleotides, siRNA, and novel nucleic acid therapeutics.
Material and connector applications: Its structural characteristics also enable potential use in optical devices and bio-conjugation.
In summary, 5'-O-DMT-N4-Bz-5-Me-dC (CAS: 104579-03-5) is not only a critical building block for efficient DNA synthesis but also a key compound bridging basic research and applied development.
